Leptin increase proliferation of EOC cells and prevents serum starved apoptosis in EOC cells. (A) SKOV3 and MDAH2774 cell lines were seeded in 96 well plates and after 24 hours serum free medium alone or with various doses of leptin as indicated was added. After 48 hours cell proliferation was measured MTT as described in materials and methods. The graph displays the mean +/-SD (standard deviation) of six independent experiments. (B) EOC cell lines were seeded in six well plates and after 24 hours serum free medium alone or 100 ng/ml was added. After 48 hours apoptosis was measured by annexin/PI staining.