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Table 4 Antibodies used for tissue micro array Immunohistochemical analysis.

From: Overexpression of leptin receptor predicts an unfavorable outcome in Middle Eastern ovarian cancer

Antibody Clone Company Source Dilution
O/N@
Retrieval Sub cellular
Localization
Detection System
Leptin-R B-3 SCBT Mouse monoclonal 1:20 O/N pH9, Pressure cooker Cytoplasmic EnVision+
Leptin Y20 SCBT Rabbit monoclonal 1:1000 O/N pH6, Pressure cooker Nuclear & Cytoplasmic EnVision+
PTEN 6H2-1 Cascade Bioscience Mouse monoclonal 1:100 pH9, Pressure cooker Cytoplasmic EnVision+
pAKT Ser473 Cell Signalling Rabbit polyclonal Predilute O/N pH9, Microwave Nuclear & Cytoplasmic Survival Marker; signal stain IHC detection kit
XIAP 48 BD Transduction Mouse monoclonal 1:300 O/N pH9, Microwave Cytoplasmic EnVision+
BCLXL 54H6 Cell Signalling Rabbit polyclonal 1:800 O/N pH9, Microwave Cytoplasmic EnVision+
pGSK3 Ser21/9 Cell Signalling Rabbit polyclonal 1:50 O/N pH9, Pressure cooker Cytoplasmic EnVision+
  1. @overnight incubation of primary antibody