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Figure 2 | Molecular Cancer

Figure 2

From: Blockage of transdifferentiation from fibroblast to myofibroblast in experimental ovarian cancer models

Figure 2

ROS-mediated up-regulation of CLIC4 expression during myofibroblast transdifferentiation. (A) CMSKOV3 and TGF-β1 induced oxidative stress in MRC-5 fibroblasts using DCF-DA analysed by fluorescent microscopy. Scale bar, 50 μm. (B, C), Myofibroblast transdifferentiation was dependent on ROS. The expression of α-SMA and CLIC4 was determined by real time RT-PCR and Western blot, using MRC-5 cells treated with or without 5 mM of antioxidant (NAC), before addition of CMSKOV3 and TGF-β1. * P < 0.05. (D), ROS mediated up-regulation of CLIC4 expression in both cytoplasm and nucleus during myofibroblast transdifferentiation. CLIC4 protein expression in cytoplasm and nucleus was determined by Western blot analysis. Fibroblasts cultured in serum-free medium served as control for all the experiments.

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