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Figure 3 | Molecular Cancer

Figure 3

From: Limited copy number - high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies

Figure 3

Detection of a KRAS mutations by LCN-HRM in a sequencing negative clinical sample. Testing of a clinical non-small cell lung cancer sample, TX450, for KRAS exon 2 by sequencing gave a negative result whereas the sample was positive by HRM. LCN-HRM was thus performed in 64 replicates using an estimate of average four copies per reaction. The difference graph plot and sequencing traces of three representatives of LCN-HRM positive reactions are shown. A KRAS c.34G>T was detected in all three reactions by subsequent sequencing.

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Molecular Cancer

ISSN: 1476-4598