Table 2 The sequence variants detected in TX86 by LCN-HRM using three different DNA polymerases.
| Polymerase | Position | Base change | Amino acid change | Reported |
|---|---|---|---|---|
|
HotStarTaq (Qiagen) | c.2515 | G>T | p.A839S | No |
| c.2519 | C>T | p.A840V | No | |
| c.2527 | G>A | p.V842I | Yes | |
| c.2532 | G>A | p.L844L | No | |
| c.2583 | G>A | p.L861L | No | |
| c.2588 | G>A | p.G863D | Yes | |
| c.2592 | G>A | p.A864A | No | |
|
FastStart High Fidelity (Roche) | c.2602 | G>A | p.E868K | No |
| c.2494 | C>T | p.R832C | No | |
| c.2512 | C>T | p.L838L | No | |
| c.2570 | G>T | p.G857V | No | |
| c.2576 | C>T | p.A859V | No | |
|
HotStar HiFidelity (Qiagen) | c.2478 | C>A | p.N826K | No |
| c.2484 | G>T | p.L828F | No | |
| c.2488 | G>T | p.D830Y | No | |
| c.2492 | G>A | p.R831H | Yes | |
| c.2494 | C>T | p.R832C | No | |
| c.2508 | C>A | p.R836R | SNP | |
| c.2508_2509 | CG>TA | p.R836R, p.D837N | No | |
| c.2509 | G>T | p.D837Y | No | |
| c.2515 | G>A | p.A839T | Yes | |
| c.2517 | A>G | p.A839A | No | |
| c.2522 | G>T | p.R841M | No | |
| c.2530 | C>A | p.L844M | No | |
| c.2543 | C>A | p.P848Q | No | |
| c.2581 | C>A | p.L861M | No | |
| c.2591 | C>T | p.A864V | No | |
| c.2607 | C>A | p.Y869X | No | |
| c.2622 | C>A | p.G874G | No |