Skip to main content

Table 3 The frequency of LCN-HRM positives measured with different DNA polymerases in a NSCLC FFPE sample, TX86.

From: Limited copy number - high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies

LCN-HRM HotStarTaq
(Qiagen)
FastStart High Fidelity
System (Roche)
HotStar HiFidelity
Polymerase (Qiagen)
Proofreading* 0 4-fold 10-fold
Positive 9 (15.5%) 5 (15.6%) 28 (52.8%)
Negative 49 (84.5%) 27 (84.4%) 25 (47.2%)
Total 58 (100%) 32 (100%) 53 (100%)
  1. * Proofreading activity was relatively compared to that of a Taq DNA polymerase. HRM: high resolution melting.