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Table 3 The frequency of LCN-HRM positives measured with different DNA polymerases in a NSCLC FFPE sample, TX86.

From: Limited copy number - high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies

LCN-HRM

HotStarTaq

(Qiagen)

FastStart High Fidelity

System (Roche)

HotStar HiFidelity

Polymerase (Qiagen)

Proofreading*

0

4-fold

10-fold

Positive

9 (15.5%)

5 (15.6%)

28 (52.8%)

Negative

49 (84.5%)

27 (84.4%)

25 (47.2%)

Total

58 (100%)

32 (100%)

53 (100%)

  1. * Proofreading activity was relatively compared to that of a Taq DNA polymerase. HRM: high resolution melting.
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Molecular Cancer

ISSN: 1476-4598