GILZ detection in epithelial ovarian cancer (EOC). (A) GILZ immunostaining in ovarian surface epithelium of normal ovaries (OSE), benign tumors, invasive serous ovarian carcinoma. Negative control was done without primary Ab. Original magnification, ×63. (B) Cytoplasmic GILZ immunostaining in clear cell, endometrioid and mucinous EOC. Original magnification, ×40. (C) GILZ immunoblots of total protein lysates from ovarian cancer tissue (representative data from several frozen tumor specimens), malignant ascites processed by the autoMacs procedure to separate EpCAM+ cells identified as epithelial cells from EpCAM- cells, from ovarian cancer cell lines SKOV-3, OVCAR-3 and BG-1. BG-1 cells stably transfected with GILZ-encoding vector pcDNA3-GILZ (pGILZ) was used as positive control (17 kDa). Actin levels are shown for normalization. Data are representative of three different experiments.