Figure 4

GILZ down-regulation reduces cell proliferation and AKT phosphorylation in BG-1 cells. BG-1 cells were transfected with 4 μg of control (siCT) or GILZ-specific (siGILZ) siRNA. (A) GILZ mRNA assayed by real-time RT-PCR and normalized to β-actin mRNA, 48 h after transfection. Results expressed as percentage of control from three independent experiments, mean ± SE. (B) Cell proliferation assayed by [³H]-thymidine incorporation 48 h after transfection with siRNA, mean ± SD, unpaired Student's t test was used for comparisons, **P < 0.01. (C) Left: total protein lysates were analyzed by western blotting using specific Abs. Blots: one representative experiment of three. Right: p-AKT expression levels were quantified by densitometric analysis and normalized to the signal for total AKT. Histogram represents mean ± SE of three experiments. Unpaired Student's t test was used for comparisons, *P < 0.05.