AKT activation is required for BG-1 cell proliferation. (A) Cell proliferation was measured by [3H]-thymidine incorporation in CTRL and pGILZ clones treated with indicated Triciribine doses for 24 h. Results are means ± SE of 3 independent experiments. (B) western blots of total protein lysates from CTRL or pGILZ clones treated with indicated Triciribine doses for 24 h. Membranes were probed with specific antibodies. The expression of GILZ, p-AKT, cyclin D1 and p21 proteins were quantified by densitometric analysis and normalized to β-actin or total AKT; results are expressed as GILZ/β-actin, p-AKT/AKT, cyclin D1/β-actin or p21/β-actin ratios. Blots: one representative experiment of three.