cell line
|
treatment
|
TSG
|
---|
| |
BEX2
|
IGSF4
|
TIMP3
|
AML-193
|
TSA
|
-
|
-
|
+
|
|
Aza
|
++
|
++
|
+++
|
|
Aza + TSA
|
++
|
++
|
+++
|
SKM-1
|
TSA
|
(+)
|
+
|
+
|
|
Aza
|
-
|
(+)
|
+
|
|
Aza + TSA
|
+
|
+
|
++
|
U-937
|
TSA
|
-
|
+
|
++
|
|
Aza
|
-
|
+++
|
+++
|
|
Aza + TSA
|
-
|
+++
|
+++
|
- Induction of TSG expression by Aza (5 μM, 4 d) and TSA (2 μM, 1 d) when compared to untreated control cells: - < 2-fold, + ≥ 2-fold; ++ ≥ 10-fold, +++ ≥ 100-fold gene expression. Expression levels were determined by quantitative real-time PCR. Note that cell line SKM-1 shows hypomethylation and expression of IGSF4 and TIMP3 (Fig. 5). Experiments were performed at least twice. Brackets: effect variable.