cell line | treatment | TSG |
---|
 |  |
BEX2
|
IGSF4
|
TIMP3
|
AML-193 | TSA | - | - |
+
|
 | Aza |
++
|
++
|
+++
|
 | Aza + TSA |
++
|
++
|
+++
|
SKM-1 | TSA |
(+)
|
+
|
+
|
 | Aza | - |
(+)
|
+
|
 | Aza + TSA |
+
|
+
|
++
|
U-937 | TSA | - |
+
|
++
|
 | Aza | - |
+++
|
+++
|
 | Aza + TSA | - |
+++
|
+++
|
- Induction of TSG expression by Aza (5 μM, 4 d) and TSA (2 μM, 1 d) when compared to untreated control cells: - < 2-fold, + ≥ 2-fold; ++ ≥ 10-fold, +++ ≥ 100-fold gene expression. Expression levels were determined by quantitative real-time PCR. Note that cell line SKM-1 shows hypomethylation and expression of IGSF4 and TIMP3 (Fig. 5). Experiments were performed at least twice. Brackets: effect variable.