Cell growth and cytotoxicity assay in HepG2 and HepG2-EGFP cell lines. (A) Growth kinetics of HepG2 and HepG2-EGFP cells shows no significant alteration in growth properties. HepG2 and HepG2-EGFP cells (2 × 103) were seeded in a 96 well plate and subsequently cultured for 24 h, 48 h, 72 h and 96 h, and MTT assay was performed. Absorbance was measured at 570 nm using 630 nm as reference filter. Optical density of 0.2 corresponds to 2 × 103 cells. (B) MMC is equally cytotoxic to HepG2 and HepG2-EGFP cells. HepG2 and HepG2-EGFP cells (1 × 104) were seeded in a 96 well plate and kept overnight for adherence. MMC treatment with indicated concentrations was given for 48 h. Cells were grown in a drug free medium for 24 h before performing the MTT assay. Data presented are representative of three independent experiments performed in triplicates and expressed as mean ± s.d.