Figure 3
From: Inhibition of succinate dehydrogenase dysregulates histone modification in mammalian cells
Functional consequences of increased histone methylation in SDHB -silenced cells. (A) Confocal images of SDHB-silenced D11 cells transiently transfected with the overexpression plasmids JMJD3 (wildtype) or JMJD3 mutated (mutant). At 72 h post transfection, cells were fixed and stained with anti-HA and anti-H3K27me3 antibodies; nuclei were stained with DAPI. Arrows indicate transfected cells. (B) Immunoblot analysis of cell lines pU6 and D11, transiently transfected for 72 h with pcDNA control (vec), JMJD3 wild-type (wt) or JMJD3 mutated (mut). Results are representative from two independent experiments. (C) ChIP analysis of H3K27me3 occupancy of the core promoter regions of IGFBP7, SNCA, PTGER, and KRT17 in pU6 and D11 cells. Gels show representative RT-PCR analysis of H3K27me3 and control IgG immunoprecipitates.