Figure 4
From: Inhibition of succinate dehydrogenase dysregulates histone modification in mammalian cells
Immunohistochemical staining for methylated histones in a sporadic carotid body paraganglioma. (A) Haematoxylin-eosin staining of a sporadic paraganglioma (100× magnification) showing typical cell nest structure. (B) Nuclear and cytoplasmic staining of sustentacular cells using an antibody directed to S100 (400×). Brown staining highlights the typical zellballen structures. (C) Nuclear staining of chief (type I) cells with the tyrosine hydroxylase marker antibody (400×). (D) Anti-H3K27me3 staining (400× with digital enlargement). A clear zellballen structure is visible, showing exclusively nuclear staining of chief cells (dark arrow) but no staining of sustentacular cells (red arrow). (E) High-magnification image of anti-H3K27me3 staining showing heterogeneous nuclear staining of chief cells (arrow). (F) Anti-H3K36me2 staining (400×). There is nuclear and some cytoplasmic staining of chief cells (dark arrow) but no nuclear staining of sustentacular cells (red arrow) and only light (background) staining of the cytoplasm. This specific staining pattern again highlights the zellballen structures. In all cases a sporadic tumour is illustrated, chosen for clarity. SDHD tumours show similar patterns of staining.