RT-PCR validation of TUSC2-dependent changes in H2595 for selected genes. A. Semi-quantitative RT-PCR analysis was done on RNA specimens used for Affymetrix profiling. Fold-estimation of up- (+) or down- (-)-regulation for each gene are shown in brackets. Invariably expressed PPIA gene served as a loading control. B. TUSC2 transcriptional targets were confirmed by semi-quantitative RT-PCR in four additional mesothelioma cell lines. TUSC2-dependent transcriptional change is shown as 'up' or 'down'; '0' indicates that PCR product was not detected.