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Figure 8 | Molecular Cancer

Figure 8

From: LMP1-augmented kappa intron enhancer activity contributes to upregulation expression of Ig kappa light chain via NF-kappaB and AP-1 pathways in nasopharyngeal carcinoma cells

Figure 8

p52/p65 and c-Jun/c-Fos heterodimers interacted with the human Ig kappa intronic enhancer and the adjacent sequence in vivo. (A) DNA sonication. 2% ethidium bromide-stained agarose gel demonstrated the shearing of chromatin from HNE2-LMP1 cells. Average chromatin length was ~500 bp. (B) ChIP analysis of the NF-κB binding site within the iEκ enhancer in HNE2-LMP1 cells. The cross-linked chromatin was precipitated with specific antibodies as indicated. The positive control was represented by the input fraction. Negative controls included a no chromatin sample, no antibody sample, and nonspecific antibody (αIgG). Precipitated DNA was analyzed by PCR using primers that amplified a 159-bp region that included the NF-κB site. (C) ChIP analysis of the AP-1 site located downstream of the iEκ enhancer. The PCR primers amplified a 188-bp sequence that included the AP-1 site.

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Molecular Cancer

ISSN: 1476-4598