Cross-talk between Aur-A and PI3K pathway regulates VX-680 induced apoptosis in TSCC cells. Serum-starved Tca8113 cells treated with IGF-1 (I, 100 ng/ml), wortmannin (W, 1 μM), VX-680 (V, 5 nM) alone or in combination for 12 h. (a) Cells were subjected to Western blot analysis with indicated antibodies. GAPDH was used as a control. (b) The apoptosis was assessed by flow cytometry as a sub-G1 population. Images showed one representative of three independent experiments. Histogram represented the quantification.