Figure 3
From: A new synthetic protein, TAT-RH, inhibits tumor growth through the regulation of NFκB activity
TAT-RH protein design and purification. A) We synthesized and purified a protein reproducing the RH domain of GRK5, TAT-RH. To this aim, RH gene was cloned into the pTAT-HA vector comprising the 11 aminoacid TAT domain, that is the minimum sequence of HIV TAT able to transduce into cells, and histidine and HA as N-terminal tags. B) The purified protein (~24 kDa) was visualized by electrophoretic analysis of samples from all steps of the purification process and gel staining. M = marker; Lane 1 = start; Lanes 2-3 = flow through; Lanes 4-6 = wash; Lanes 7-9 = imidazole.