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Figure 4 | Molecular Cancer

Figure 4

From: A new synthetic protein, TAT-RH, inhibits tumor growth through the regulation of NFκB activity

Figure 4

Biological effects of TAT-RH in KAT-4 cells. A) To evaluate the ability of the recombinant protein to autonomously enter into cells, we added TAT-RH (0,5 μg/ml) for 1 hour to culture medium and prepared cell lysates after three washes with saline buffer. We then evaluated the presence of TAT-RH in cell lysates by means of histidine precipitation and HA analysis by western blot. The purified protein was used as positive control. B) To verify the biological effect of TAT-RH, we evaluated IκBα and cleaved caspase 3 levels which were both increased by treatment. Actin was used as control. C) Apoptosis was also analized by Annexin V staining. TAT-RH increases Annexin V staining compared to controls (green = Annexin V; blue = nuclei).

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