Figure 6

Effects of TAT-RH in vivo on tumor growth and biochemical analysis. A) We evaluated the effects of TAT-RH on tumor growth in BALB/c nude mice. High doses (16 mg/kg in 200 μl) lead to regression of tumors, intermediate doses (12 mg/kg in 200 μl) are able to completely inhibit tumor growth and low doses (8 mg/kg in 200 μl) can only delay tumor growth. Figure also shows a representative image of tumors at the end of the treatment (inset). B) Tumors were homogenized and the biological effects of TAT-RH treatment were evaluated by WB. TAT-RH leads to a dose-dependent increase of IκBα and cleaved caspase 3 and a reduction of VEGF levels. TAT treated tumors were used as controls. C) To evaluate the effect of TAT-RH on NFκB activity, we performed an EMSA using nuclear extracts from tumors. TAT-RH treatment reduces NFκB activity. Lane 1 = probe alone; Lane 2 = empty; Lane 3-4 = TAT treated tumors; Lane 5-6 = TAT-RH treated tumors.