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Figure 4 | Molecular Cancer

Figure 4

From: Insulin-like growth factor binding protein-3 has dual effects on gastrointestinal stromal tumor cell viability and sensitivity to the anti-tumor effects of imatinib mesylate in vitro

Figure 4

Effect of IGFBP3 neutralization or knockdown on GIST882 response to imatinib. (A) Cells were treated simultaneously with imatinib (1 μM) and anti-IGFBP3 or control IgG (4 μg/mL) for 48 hours, and viability was assessed with the MTS assay. (B) RNA was extracted from ST882 cells after transfection with of siRNA (50 or 100 nM) and imatinib (1 μM) for 48 hours. After the reverse transcriptase reaction, IGFBP3 mRNA levels were quantified by real-time PCR. (C)Whole cell lysate isolated after siRNA (50 nM) transfection and imatinib (1 μM) treatment were analyzed for IGFBP3 expression by immunoblotting. (D) Viability after siRNA (50 nM) transfection and imatinib treatment (1 μM) was assessed by MTS.

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Molecular Cancer

ISSN: 1476-4598