Figure 10
RT-PCR analysis for (A) the interleukin-1 receptors 1 and 2 (IL1R1 and IL1R2), TNF receptors 1 and 2 (TNFR1 and TNFR2) in Ad/AH and Hone1 cells stably expressing EBNA1 or a neomycin control vector (neo) indicate that EBNA1 does not alter the natural level of expression of IL-1 or TNF receptors in carcinoma cells. (B) RT-PCR analysis for IL-1α, C/EBPβ and A20 in Ad/AH and Hone1 cells stably expressing EBNA1 or a neomycin control vector (neo) indicate that EBNA1 does not inhibit NF-κB activity through down-regulation of pro-inflammatory cytokines or up-regulation of C/EBPβ or A20. RT-PCR for EBNA1 and GAPDH serve as EBNA1 and loading controls, respectively, whilst the water only sample (H2O) serves as a general PCR control.