Figure 3

EMSA analysis with quantitative densitometry (lower panel) demonstrating that stable EBNA1 expression in Ad/AH cells inhibits basal NF-κB DNA binding and in response to TNFα, relative to Ad/AH cells expressing a neomycin control plasmid (neo) (upper). Cold competition using a 100-fold excess of unlabelled NF-κB probe (cold competitor) ablates NF-κB binding (middle). EMSAs were performed in triplicate.