Skip to main content
Figure 9 | Molecular Cancer

Figure 9

From: Epstein-Barr virus-encoded EBNA1 inhibits the canonical NF-κB pathway in carcinoma cells by inhibiting IKK phosphorylation

Figure 9

NF-κB luciferase reporter activity following transient transfection of Ad/AH cells with concentrations of lentivirus vectors that result in equal levels of expression of wild-type EBNA1 and mutants. Wild-type (wtEBNA1), gly-ala repeat-deleted EBNA1 (dGA) and EBNA1 deleted for the cellular USP7 binding site (d395-450 dUSP7) result in significant inhibition of NF-κB reporter activity, relative to the empty vector control (Vector). In contrast, deletion of EBNA1 domains (d8-67, d41-376, d61-83 and d325-376) characterised as being essential for the transactivation of EBV genes does not result in inhibition of NF-κB reporter activity. Reporter assays were performed in biological and technical triplicate and error bars indicate SD (* = P < 0.05 relative to empty vector control).

Back to article page

Molecular Cancer

ISSN: 1476-4598