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Table 2 Antibodies used in immunoblotting (IM), immunofluorescence (IF) and immunohistochemistry (IHC)

From: Epstein-Barr virus-encoded EBNA1 inhibits the canonical NF-κB pathway in carcinoma cells by inhibiting IKK phosphorylation

Protein Primary antibody Species Application
β-actin Sigma-Aldrich (A5441) Mouse IM
EBNA1 A.M. Human sera IM
EBNA1 R4 Rabbit IF
IKKα Santa Cruz (sc-7607) Mouse IM
IKKβ Cell signalling (L570) Rabbit IM
IKKγ Santa Cruz (sc-8330) Rabbit IM
IκBα Cell signalling (9242) Rabbit IM
p65/RelA Cell signalling (3034) Rabbit IM
p65/RelA Santa Cruz (sc-372) Rabbit IF, IHC
Phospho-IκBα (Ser32)
Cell signalling (2859)
Rabbit IM
Phospho-IKKα/β (Ser176/180)
Cell signalling (2687)
Rabbit IM
Phospho-p65 (Ser536)
Cell signalling (3031)
Rabbit IM