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Figure 3 | Molecular Cancer

Figure 3

From: Osteopontin selectively regulates p70S6K/mTOR phosphorylation leading to NF-κB dependent AP-1-mediated ICAM-1 expression in breast cancer cells

Figure 3

mTOR suppresses OPN-induced NF-κB Activation. A and B. MCF-7 cells were treated with 0.5 μM OPN for 0-240 min or transiently transfected with wild type or rapamycin resistant mTOR and then treated with OPN. The non-transfected or transfected cells were treated with rapamycin alone or along with OPN under the same condition as described in Materials and Methods. Nuclear extracts were prepared and analyzed by EMSA. C. MCF-7 cells were transfected with wt or rapamycin resistant mTOR along with NF-κB luciferase reporter construct and then treated with 0.5 μM OPN. The non-transfected or transfected cells were treated with rapamycin alone or along with OPN. The transfection efficiency was normalized by Renilla and fold changes in luciferase activity with respect to control were calculated, and mean ± S.D. of triplicate determinations are plotted. The values were also analyzed by One Way ANOVA (*, p < 0.05; **, p < 0.05).

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Molecular Cancer

ISSN: 1476-4598