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Figure 7 | Molecular Cancer

Figure 7

From: Osteopontin selectively regulates p70S6K/mTOR phosphorylation leading to NF-κB dependent AP-1-mediated ICAM-1 expression in breast cancer cells

Figure 7

OPN induces mTOR independent p70S6 kinase phosphorylation at Thr-421/Ser-424 through MEK/ERK pathway. A. MCF-7 cells were transiently transfected with wild type or rapamycin resistant mTOR. After 48 h, cells were either treated with OPN or pretreated with rapamycin and then treated with 0.5 μM OPN for 10 min. Cell lysates containing equal amount of total proteins were analyzed by western blot with anti-p-p70S6 kinase (Thr-421/Ser-424) or anti-mTOR antibody. The blots were reprobed with anti-p70S6 kinase antibody. B. MCF-7 cells were pretreated with 0-500 μM U0126 (MEK inhibitor) for 1 h and then treated with 0.5 μM OPN for 10 min. Cell lysates were analyzed by western blot with anti-p-p70S6 kinase (Thr-421/Ser-424) or anti-p-ERK1/2 antibody. The same blots were reprobed with anti-p70S6 kinase or anti-ERK2 antibody.

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Molecular Cancer

ISSN: 1476-4598