Figure 4From: Cyclin K and cyclin D1b are oncogenic in myeloma cellsCyclin K and cyclin D1b impact the biology of LP-1 cells. Proteins from exponentially growing cells were resolved by SDS-PAGE and immunoblotted with the following Abs: anti-cyclin D2 (sc-181), anti-CDK2 (sc-6248), anti-p15 (sc-612), anti-p18 (sc-865), anti-β-tubulin (sc-9104) from Santa Cruz Biotech.; anti-p53 (Ab-1, Calbiochem, Merck Chemicals Ltd., Nottingham, UK); anti-p44/42 MAPK (#9102), anti-phospho-p44/42 MAPK (Thr202/Tyr204) (#9101), anti-p70S6K (#9202), anti-phospho-p70S6K (Thr389) (#9205), anti-AKT (#9272), anti-phospho-AKT (Thr308) (#4055), anti-Stat3 (#9132), anti-phospho-Stat3 (Ser727) (#9134, Cell Signaling Technology, Danvers, MA, USA). Blots were reprobed with an anti-β-tubulin Ab as control of charge and transfer.Back to article page