Figure 6

Cyclin D1b promotes tumor growth by stimulating angiogenesis. a) Fixed tumor sections were studied with conventional IHC for CD34 (brown staining) expression. Anti-CD34 Ab (MEC14.7) was purchased from Hycult Biotech. (Uden, The Netherlands). Images (40× magnification) are representative of 3 experiments performed on 3 different tumors. b) Fertile eggs were incubated at 37.8°C and 80% humidity. On day 10, LP-1K and LP-1D1b cells (2 × 10⁷ cells per egg) were inoculated on the CAM. At days 2, 4 and 6 after cells implantation, digital images of primary tumors were acquired at ×7.5 magnification. c) Five mice per condition were inoculated s.c. with LP-1D1b cells. For siRNA experiments, mice were injected the day after with a mixture of AteloGene™ and scrambled (green square) or VEGF (blue circle) siRNA. For chemical inhibitors experiments, mice were injected biweekly starting at day 1 following cell injection with SSR (purple circle) or SAR (red diamond). Non treated mice were used as controls (black triangle). The volume of tumors was measured at day 11 and during the period indicated and the tumor growth evaluated. The groups of mice injected with VEGF siRNA and untreated were compared at days 11 and 15; *, p < 0.05.