Expression of FOXA 1, FOXA2, and β-catenin significantly increase ICK reporter activity. HEK-293T cells in 12-well plates were transiently co-transfected with the ICK-7 luciferase reporter, the control CMV-β-galactosidase reporter, and with expression vectors encoding the various transcription factors or vector control (VC) essentially as described for NFκB reporter assays . The β-galactosidase activities were used to normalize the luciferase values. Co-transfections were also performed with the promoterless ICK-9 luciferase reporter, which served as a negative transcription control. Western blot analyses were performed to ensure that cells expressed the transcription factors (see additional file 1). No suitable antibody was available for CDX1. Data represents the mean + SD of two independent experiments performed in triplicate.