TSP1 mRNA induction by tasquinimod in in vitro tumor cell cultures. Tasquinimod-induced mRNA expression in LNCaP cells measured by real time semi-quantitative RT-PCR. (A) Time study after in vitro exposure with 10 μM tasquinimod, and (B) dose response with tasquinimod treatment between 0.1-100 μM for 72 h (p = 0.0128, ANOVA). Data expressed represents the mean ± SD of at least two independent experiments. (*) p ≤ 0.05 and (**) p ≤ 0.01 compared with untreated control (Bonferroni's multiple comparison test).