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Table 2 Primer and probe sets used for RT-PCR and semi-quantitative real time PCR*.

From: Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors

Target gene Primer
Sequence (5' => 3')
Probe
Sequence (5' => 3')
Amplicon length (bp)
hu GAPDH FW: gAAggTgAAggTCggAgTC
Rev: gAAgATggTgATgggATTTC
AggggTCATTgATggCAACAATATCCA-FL
LC:640-TTTACCAgAgTTAAAAgCAgCCCTggTg
226
hu TSP-1 FW: CTggACTCgCTgTAggTTA
Rev: CCCTgTggTggAgTTTAC
AgTCATCgTCCCTTTCggTg-FL
LC: 640-TgATgAAgAAggTgCCACTgAAgT
233
hu CXCR4 FW: CgAggAAATgggCTCAg
Rev: gggAAgCgTgATgACAAA
TTTTATTgAAATTAgCATTTTCTTCACggA-FL
LC:640-ACAgggTTCCTTCATggAgTCATAgTCC
273
hu CYP1A1 FW: gAgCTgggTTTgACACAgTC
Rev: ggATgTAAAAgCCTTTCAAACT
gTCggAAggTCTCCAggATgAAg-FL
LC: 640-CCTCCATATAgggCAgATgggATCTg
258
hu GDF15 FW:gAAgACTCCAgATTCCgAgAgTT
Rev:gATCCCAgCCgCACTTCT
gATTCgAACACCgACCTCgTCCC-FL
LC: 640-gCCCCTgCAgTCCggATACTCAC
146
hu AGER FW: TCTgCCTCTgAACTCACgg
Rev: CCTTCACAgATACTCCCTTCTCATT
CAgggACTCTTAgCTggCACTTgg--FL
LC: 640-TgggAAAgCCCCTggTgCCT
139
huTSP-1 FW: AACggAgTTCAgTACAgAAAT
Rev: TTCCATTgCCACAgCTC
CAgAACTCRgTTACCATCTgCAAAAAgg-FL
LC: 640-gTCCTgYCCCATCATgCCCTgC
235
mTsp-1 FW: AgAgAACAgAgAgCTggTCAg
Rev: ATCTgTTgTTgAggCTgTCA
  329
mPbgd FW:TTgTACCCTggCATACAgTTTgA
Rev: gTTCCCACggCACTTTTC
TgAAggATgTgCCTACCATACTACCTCCT-FL
LC: 640-gCTTTACTATTggAgCCATCTgCAAAC
247
  1. * Designed, synthesized and purified by TIB MOLBIOL (Berlin, Germany)
  2. Probes designated LC contain the acceptor dye LigtCycler Red 640 covalently attached at the 5'-end, and the FL probes contain the donor dye Fluorescein at the 3'-end.
  3. Specific mouse- and human TSP1 primers sharing the same LC/FL probe sequence for TSP1.