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Table 2 Primer and probe sets used for RT-PCR and semi-quantitative real time PCR*.

From: Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors

Target gene Primer Sequence (5' => 3') Probe Sequence (5' => 3') Amplicon length (bp)
hu GAPDH FW: gAAggTgAAggTCggAgTC Rev: gAAgATggTgATgggATTTC AggggTCATTgATggCAACAATATCCA-FL LC:640-TTTACCAgAgTTAAAAgCAgCCCTggTg 226
hu TSP-1 FW: CTggACTCgCTgTAggTTA Rev: CCCTgTggTggAgTTTAC AgTCATCgTCCCTTTCggTg-FL LC: 640-TgATgAAgAAggTgCCACTgAAgT 233
hu CXCR4 FW: CgAggAAATgggCTCAg Rev: gggAAgCgTgATgACAAA TTTTATTgAAATTAgCATTTTCTTCACggA-FL LC:640-ACAgggTTCCTTCATggAgTCATAgTCC 273
hu CYP1A1 FW: gAgCTgggTTTgACACAgTC Rev: ggATgTAAAAgCCTTTCAAACT gTCggAAggTCTCCAggATgAAg-FL LC: 640-CCTCCATATAgggCAgATgggATCTg 258
hu GDF15 FW:gAAgACTCCAgATTCCgAgAgTT Rev:gATCCCAgCCgCACTTCT gATTCgAACACCgACCTCgTCCC-FL LC: 640-gCCCCTgCAgTCCggATACTCAC 146
hu AGER FW: TCTgCCTCTgAACTCACgg Rev: CCTTCACAgATACTCCCTTCTCATT CAgggACTCTTAgCTggCACTTgg--FL LC: 640-TgggAAAgCCCCTggTgCCT 139
huTSP-1 FW: AACggAgTTCAgTACAgAAAT Rev: TTCCATTgCCACAgCTC CAgAACTCRgTTACCATCTgCAAAAAgg-FL LC: 640-gTCCTgYCCCATCATgCCCTgC 235
mTsp-1 FW: AgAgAACAgAgAgCTggTCAg Rev: ATCTgTTgTTgAggCTgTCA   329
mPbgd FW:TTgTACCCTggCATACAgTTTgA Rev: gTTCCCACggCACTTTTC TgAAggATgTgCCTACCATACTACCTCCT-FL LC: 640-gCTTTACTATTggAgCCATCTgCAAAC 247
  1. * Designed, synthesized and purified by TIB MOLBIOL (Berlin, Germany)
  2. Probes designated LC contain the acceptor dye LigtCycler Red 640 covalently attached at the 5'-end, and the FL probes contain the donor dye Fluorescein at the 3'-end.
  3. Specific mouse- and human TSP1 primers sharing the same LC/FL probe sequence for TSP1.