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Table 2 Primer and probe sets used for RT-PCR and semi-quantitative real time PCR*.

From: Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors

Target gene

Primer

Sequence (5' => 3')

Probe

Sequence (5' => 3')

Amplicon length (bp)

hu GAPDH

FW: gAAggTgAAggTCggAgTC

Rev: gAAgATggTgATgggATTTC

AggggTCATTgATggCAACAATATCCA-FL

LC:640-TTTACCAgAgTTAAAAgCAgCCCTggTg

226

hu TSP-1

FW: CTggACTCgCTgTAggTTA

Rev: CCCTgTggTggAgTTTAC

AgTCATCgTCCCTTTCggTg-FL

LC: 640-TgATgAAgAAggTgCCACTgAAgT

233

hu CXCR4

FW: CgAggAAATgggCTCAg

Rev: gggAAgCgTgATgACAAA

TTTTATTgAAATTAgCATTTTCTTCACggA-FL

LC:640-ACAgggTTCCTTCATggAgTCATAgTCC

273

hu CYP1A1

FW: gAgCTgggTTTgACACAgTC

Rev: ggATgTAAAAgCCTTTCAAACT

gTCggAAggTCTCCAggATgAAg-FL

LC: 640-CCTCCATATAgggCAgATgggATCTg

258

hu GDF15

FW:gAAgACTCCAgATTCCgAgAgTT

Rev:gATCCCAgCCgCACTTCT

gATTCgAACACCgACCTCgTCCC-FL

LC: 640-gCCCCTgCAgTCCggATACTCAC

146

hu AGER

FW: TCTgCCTCTgAACTCACgg

Rev: CCTTCACAgATACTCCCTTCTCATT

CAgggACTCTTAgCTggCACTTgg--FL

LC: 640-TgggAAAgCCCCTggTgCCT

139

huTSP-1

FW: AACggAgTTCAgTACAgAAAT

Rev: TTCCATTgCCACAgCTC

CAgAACTCRgTTACCATCTgCAAAAAgg-FL

LC: 640-gTCCTgYCCCATCATgCCCTgC

235

mTsp-1

FW: AgAgAACAgAgAgCTggTCAg

Rev: ATCTgTTgTTgAggCTgTCA

 

329

mPbgd

FW:TTgTACCCTggCATACAgTTTgA

Rev: gTTCCCACggCACTTTTC

TgAAggATgTgCCTACCATACTACCTCCT-FL

LC: 640-gCTTTACTATTggAgCCATCTgCAAAC

247

  1. * Designed, synthesized and purified by TIB MOLBIOL (Berlin, Germany)
  2. Probes designated LC contain the acceptor dye LigtCycler Red 640 covalently attached at the 5'-end, and the FL probes contain the donor dye Fluorescein at the 3'-end.
  3. Specific mouse- and human TSP1 primers sharing the same LC/FL probe sequence for TSP1.