Figure 1
From: The anti-aging gene KLOTHO is a novel target for epigenetic silencing in human cervical carcinoma
Transcriptional repression of KLOTHO is associated with epigenetic inactivation in cervical cancer. A, RT-PCR analysis showing mRNA expression of the secreted Frizzled-related protein (SFRP) class of secreted Wnt antagonists in cervical cancer cell lines. Overall downregulation of SFRPs and variable mRNA levels of KLOTHO (KL) were detected. ACTB (encoding β-actin) is shown as an internal control. B, The KLOTHO mRNA expression pattern in primary cervical tumor samples. cDNAs prepared from human placenta (Plc) and from normal (N) cervical tissue were used as positive controls for KLOTHO expression. C, Transcriptional restoration of KLOTHO by either the DNMT inhibitor DAC or the HDAC inhibitor TSA in representative non-KLOTHO-expressing cell lines.