Figure 5
From: The anti-aging gene KLOTHO is a novel target for epigenetic silencing in human cervical carcinoma
Inhibition of the Wnt/β-catenin pathway and colony growth following ectopic expression of the secreted form of KLOTHO (sKL) in a cervical cancer cell line. A, Immunoblot analysis of KLOTHO, total β-catenin, and active β-catenin (ABC) in CaSki cells transfected with either the sKL expression vector or the empty vector. B, RT-PCR analysis of TCF/β-catenin target genes, i.e., c-MYC and CCND1, in CaSki cells transfected with either the sKL expression vector or the empty vector. ACTB is shown as an internal control. C, Colony formation assay showing that restoration of KLOTHO suppresses tumor cell growth in the CaSki cell line.