Figure 3
From: MUC16 provides immune protection by inhibiting synapse formation between NK and ovarian tumor cells
Expression of mucins and NK cell activating and inhibitory molecules on csMUC16neg-OVC and csMUC16pos-OVC. MUC1 expression on both sublines is similar and MUC4 expression is absent. Expression of DNAM-1 and NKG2D ligands on the sublines was determined by staining with Fc chimeras (15 μg/mL) of these two receptors followed by labeling with fluorophore conjugated anti-Fc secondary antibody. HLA class I expression on the sublines was determined by labeling with FITC conjugated w6/32. Histograms of live single events are shown in all plots. Data is representative of three independent experiments. Shaded peaks show control cells incubated with only the fluorescently-tagged secondary antibodies. Un-shaded histograms are for cells incubated with the primary antibodies or Fc chimeras and the fluorescently labeled secondary antibodies.