Figure 4
From: MUC16 provides immune protection by inhibiting synapse formation between NK and ovarian tumor cells
csMUC16 protects ovarian cancer cells from lysis by NK cells. NK cells from four healthy donors were isolated and 51Cr release lysis assays at 10:1, 5:1, and 1:1 effector:target ratios were conducted using csMUC16neg-OVC and csMUC16pos-OVC cells. Chromium release from the targets was determined after 4 h incubation under standard tissue culture conditions. Percent lysis was calculated by determining the spontaneous and maximum release of radioactivity from each cell line.