Figure 4

Transition from S-phase to G2/M is Blocked in 8505C Cells in Response to NF-κB Inhibition. (A) 8505C cells were transduced with either Ad-GFP vs. Ad-mIκBα at an MOI of 25. After 48 hours, cells were harvested, and permeabilized. DNA was stained with a saponin/propidium iodide solution and subjected to cell cycle analysis by flow cytometry. Data is represented as relative cell number (%) in G1, S, or G2/M. Mean ± S.E.M. of 2 independent experiments performed in duplicate is reported. [p < 0.05 (*); p < 0.01 (**); p < 0.001 (***)] (B) 8505C cells were transduced with either Ad-GFP or Ad-mIκBα at an MOI of 25. Whole-cell lysates were prepared 24 and 48 hours post-transduction, and levels of cyclin A, cdc2, phospho-cdc2, cyclin B1, and p21 were assessed by Western blot analysis. α-tubulin was used as a loading control.