Figure 6

Sustained Activation of the JNK Pathway Predicts Sensitivity to the Combinatorial Effects of Genetic NF-κB Inhibition and Treatment with TNFα. BCPAP, SW1736, TPC1, and C643 cells were transduced with either Ad-GFP or Ad-mIκBα at an MOI of 200, 100, 50, and 100, respectively. Whole-cell extracts were harvested after 0.5, 1, 2, 4, 8 and 16 hours. Western blot analysis was performed to assess levels of ppJNK 1/2 (Thr-183/Tyr-185) and JNK 1/2. α-tubulin was used as a loading control. n.s. denotes a non-specific band (more prominent in BCPAP and C643 cells).