Figure 4
From: Identification of Siglec-9 as the receptor for MUC16 on human NK cells, B cells, and monocytes
sMUC16 predominantly binds to Siglec-9pos B cells and monocytes. B cells (CD19pos) and monocytes (CD33pos) present in the PBMC of healthy donors (HD) and PBMC and PFMC of ovarian cancer patients (PT) were identified by flow cytometry. A, Siglec-9 expression and sMUC16 binding on B cells was determined and double positive events were gated. Data shown is mean of percent positive events for six HD and five PT PBMC samples. B, High level of expression of Siglec-9 and a correspondingly high level of sMUC16 binding was observed on monocytes. Data shown is for HD #29 and PT #36 and is representative of the cumulative data, C, obtained for six HD and five PT.