Figure 6
From: Identification of Siglec-9 as the receptor for MUC16 on human NK cells, B cells, and monocytes
sMUC16-Siglec-9 binding specificity. A, Siglec-7 (top panel) and Siglec-9 (bottom panel) expressing Jurkat cells were incubated for 24 h in media, media containing OVCAR-3 derived sMUC16 (50,000 U of CA125/ml), or in 10% media containing 90% peritoneal fluid (PF) from ovarian cancer patients (PT #30 and PT #42). The VK-8 antibody was used to detect binding of sMUC16 to the cells by flow cytometry. B, Siglec-9 expressing Jurkat cells were incubated with native and partially desialylated sMUC16 (50,000 U of CA125/ml) for 3 h. Cells were labeled with VK8 and the amount of sMUC16 present on the surface was determined by flow cytometry. Mean and standard deviation of three separate experiments (p < 0.05) is shown.