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Figure 1 | Molecular Cancer

Figure 1

From: Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents

Figure 1

Detection of monoubiquitinated (L) and non-monoubiquitinated (S) FANCD2 protein to assess proximal FA pathway status in GI cell lines. (A) Immunoblotting to assess FANCD2 monoubiquitination in 48 tumor cell lines of pancreatic, colorectal, hepatocellular, gastric and cholangiocellular origin. Pancreatic cell lines were described previously, including the FA pathway-deficient cell lines Hs766T and PL11 (small arrows), and served as controls. The HCC cell line HuH-7 (big arrow) lacked spontaneous FANCD2 monoubiquitination. (B) FANCD2 immunoblotting of RKO cells (positive control), derived RKO FANCG -/- cells (negative control) and HuH-7 cells upon treatment with MMC at 100 nM for 24 h to stimulate potential previously undetectable FANCD2 monoubiquitination.

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Molecular Cancer

ISSN: 1476-4598