Figure 2

AT-MSC abrogated tumor dormancy of low melanoma cell dose and supported tumor growth. 1 × 10⁵ or 1 × 10⁴ A375 cells resuspended in serum-free culture media were injected s.c. either alone or admixed to AT-MSC in ratios 10:1, 1:1 or 1:10. Low A375 cell dose (1 × 10⁵ s.c.) resuspended in cell-and-serum free AT-MSC-conditioned media was injected in a separate groups of animals. A. AT-MSC admixed to the A375 at a ratio 1:1 or 10:1 significantly increased tumor incidence in nude mice. AT-MSC conditioned media also abrogated tumor dormancy of low A375 melanoma dose. B. Tumor burden in 10:1 and 1:1 AT-MSC coinjected groups was significantly higher in comparison to A375 cell-induced xenografts by day 40. C. AT-MSC co-administration resulted in melanoma xenograft tumor growth support proportional to the AT-MSC cell dose (*P < 0.05).