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Figure 4 | Molecular Cancer

Figure 4

From: Tumor cell behaviour modulation by mesenchymal stromal cells

Figure 4

Paracrine signalling in indirect AT-MSC/A375 co-cultures. A. AT-MSC increased colony-formation by A375 cells. A375 cells were plated in the lower well part and combined with insert containing AT-MSC, fibroblast or no cells in upper part for 9 days in indirect coculture in vitro. Average number of A375 colonies/bottom well is shown in one representative experiment performed in triplicates (*P < 0.05). B. Quantitative RT-PCR was performed on templates isolated from indirectly cocultured A375 and AT-MSC cells. Gene expression level was compared to parental cells and expressed as relative gene expression. HGF and cMet expression is sustained upon coculture, CCL5 increases in AT-MSC cells cocultured with A375 cells for 3 days. C. Semi-quantitative expression analysis of parental A375, AT-MSC and cocultured A375 and AT-MSC cells confirms sustained expression of EGFR, VEGF A, VEGF B, VEGFR1, PDGF-bb, SDF-1α, SCF, CCL5, HGF, cMet in AT-MSC; and EGFR, VEGF-A,-B, VEGFR-1,-2 PDGF-bb, SDF-1α, CCL5, HGF, cMet in A375.

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