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Figure 5 | Molecular Cancer

Figure 5

From: Tumor cell behaviour modulation by mesenchymal stromal cells

Figure 5

Direct AT-MSC/A375 interactions synergistically increase VEGF production and its neutralization can partially inhibit AT-MSC mediated tumor onset and growth support. A375, AT-MSC, or A375/AT-MSC were directly co-cultured in complete media for 3 days. A375/AT-MSC ratio was A) 2:1 or B) 5:1. The level of cytokines in cell-free supernatants was determined by Bio-Plex cytokine arrays and normalized to the levels observed in the media of A375 cell cultured alone. Mostly, the effects were additive or slightly synergistic. The levels of G-CSF and VEGF were significantly increased in both cases and exhibited potent synergistic effect. Data were expressed as average fold induction. ND, not detected. C) 2 × 105 A375 cells resuspended in serum-free culture media were injected s.c. either alone or admixed to AT-MSC in ratio 2:1. One group of animals was treated with 1 mg/kg Avastin twice a week as indicated (antiVEGF group). Left panel: AT-MSC admixed to the A375 at a ratio 2:1 significantly increase tumor growth in nude mice and abrogate tumor dormancy (p = 0.0013). VEGF neutralization significantly inhibited protumorigenic AT-MSC mediated effect (p = 0.0498). Right panel: AntiVEGF treatment significantly decreased tumor burden and counteracted protumorigenic effects of AT-MSC (*p < 0.05).

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