Gene expression profiling of cholangiocarcinoma-derived fibroblast reveals alterations related to tumor progression and indicates periostin as a poor prognostic marker

Table 7 Primer sequences for real time PCR.

Gene	Forward Primer	Reverse Primer	Size	Accession no.
	5'-3'	5'-3'	(bp)
ADAM12	tttgggggtcaacagttttc	agagctgggttcccttttgt	191	NM_003474
AREG	tggggaaaagtccatgaaaa	tttcgttcctcagcttctcc	174	NM_001657
AGN2	ccacctgaggaactgtctcg	ggtcttgctttggtccgtta	191	NM_001147
ER	catatgggagaagggggagt	aagtgcaattacagagtgcaaaa	166	NM_001432
JAGL1	gcctgccttaagtgaggaaa	gccaagaacaacacatcaaaga	169	U77914
LAMA5	gtgatgaaaagcgggaatgt	acctccacagagcgagtcat	221	BC003355
NOV	tgcaattccaagaaaatatcactg	cttggatttggagcttggaa	167	NM_002514
PDGF-A	acacgagcagtgtcaagtgc	tctggttggctgctttaggt	250	X03795
PN	cactctttgctcccaccaat	tcaaagactgctcctcccata	157	AY140646
RL	tgctgaatttggggctactt	gggagatagggtcttcatcca	198	NM_005045
SCG2	cccgaagaatgatgataccc	aaatgttgggatttgcttgg	195	NM_003469
ITGα ₅	agttgcatttccgagtctgg	ccaaacaggatggctaggat	223	NM_002205
β-actin	cacactgtgcccatctacga	ctccttaatgtcacgcacga	162	X00351
gapdh	ctcctcctgttcgacagtca	gttaaaagcagccctggtga	140	NM_002046

Note: ADAM12, a disintegrin and matrix metalloproteinase 12; AREG, amphiregulin; AGN2, angiopoietin 2; ER, epiregulin; JAGL1, jagged soluble form; LAMA5, laminin alpha 5; NOV, nephroblastoma over expressed; PDGF-A, platelet-derived growth factor alpha; PN, periostin; RL, reelin; SCG2, secretogranin 2; ITGα5, integrin alpha 5; β-actin, beta-actin; gapdh, glyceraldehyde 3-phosphate dehydrogenase

ISSN: 1476-4598