Figure 3
From: TGFBI expression is associated with a better response to chemotherapy in NSCLC
TGFBI sensitizes NSCLC cells to chemotherapy. A549 cells transfected with TGFBI siRNA plasmid (TGFBIsi) (A), and H1299 cells transfected with TGFBI expression vector (TGFBIve) (B), were exposed to increasing amounts of different cytotoxic agents for 48 h and cell viability was measured. Three independent experiments were performed. Comparisons to analyze statistical differences between control and transfected cells were performed using Student t-test (* p < 0.05; ** p < 0.01). IC50 for each experimental condition is indicated. (C, D) PARP-1 cleavage was detected by Western blot analysis of total protein extracts derived from A549-TGFBIsi (C) and H1299-TGFBIve (D) transfected cells treated with etoposide for 48 h. One representative experiment out of three is shown.