Figure 5
From: TGFBI expression is associated with a better response to chemotherapy in NSCLC
TGFBI influences NSCLC susceptibility to chemotherapy through binding to the αvβ 3 integrin. Adhesion of calcein-labeled A549 (A) and H1299 (B) NSCLC cells to rh-TGFBI coated (1 μg/mL) 96-well plates was evaluated in the presence of antibodies against several integrins. Statistical analyses were performed using Student t-test and comparing treated cells to control cells (**p < 0.01). (C) Flow cytometry analysis of basal αvβ3 integrin expression in A549 cells (dashed histogram), H1299 cells (line histrogram) or isotype control (dotted histogram). (D) Caspase 3/7 detection in A549 and H1299 cells seeded onto non-coated 96 well-plates, pre-incubated for 1 h with anti-αvβ3 monoclonal blocking antibody, exposed to rh-TGFBI (20 μg/mL) for 24 h and further treated with etoposide at the IC50 (5 μM for A549 cells and 50 μM for H1299 cells) for additional 24 h. Statistical comparisons were performed using Student t-test. **p < 0.01 for the comparison of the activity control cells versus integrin blocked cells. ##p < 0.01 for the comparison of the activity of etoposide-treated cells versus cells treated in the presence of the anti-integrin blocking antibody.