Reptin depletion severely impairs the clonogenic potential and growth of gastric cancer cells. (A) Cells were transfected with Reptin siRNA for 72 hours and then seeded into six-well plates (300 cells/well). After ten days, Giemsa staining was performed and the foci number was documented. Six independent experiments were performed. (B) AGS and HGC-27 cells treated with Control and Reptin siRNA were analyzed for cell cycle distribution using flow cytometry with ModFit. Two independent experiments were performed with similar results.