Figure 1

Real-time RT-PCR analysis of mature miR-143 and - 145 expression in human normal tissues. Total RNAs from human normal tissues were purchased from Biochain (Hayward, USA) and Takara (Otsu, Japan). Expression of both miRNAs in each sample was detected by using a TaqMan microRNA reverse transcription kit and TaqMan microRNA Assay Kit (Applied Biosystems, Foster City, USA) and normalized with RNU6B. Calculation of the Ct value was done by using the second derivative maximum method, and relative quantification was analyzed by the comparative Ct (ΔCt) method. All reactions were run in triplicate. Relative miR-143 (A) and -145 (B) expression levels (value of 2^{-ΔCt(miR-RNU6B)}) are indicated on the left axis, with error bars indicating the standard deviation for these analyses.