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Figure 4 | Molecular Cancer

Figure 4

From: Targeting melanoma growth and viability reveals dualistic functionality of the phosphonothionate analogue of carba cyclic phosphatidic acid

Figure 4

Knockdown of the LPA3 receptor induces cell death in A375 melanoma cells. (A) LPA 18:1 treatment (0.1-10 μM) of A375 cells in serum free medium for 48 h enhances viability. *p < 0.001 vs. untreated control. (B) A375 cells were transfected for 48 h with the indicated siRNAs and assessed for membrane integrity and cell death using trypan blue exclusion assay. Cell numbers reflect the cells with intact membranes. *P < 0.001, comparing siRISC 60 nM vs. treatment conditions. (C) Transfection of siRNA targeting the LPA receptors in A375 cells for 48 h demonstrates that reducing the expression of the LPA3 receptor decreases the number of live cells assessed by crystal violet staining. *p < 0.05 vs. control. (D) Photomicrograph images demonstrating changes in cell morphology after 48 h of siLPA3 transfection. (E) RT-PCR demonstrating the level of expression of ATX and GPR87 in fetal skin, normal human skin, A375 and MeWo cells.

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Molecular Cancer

ISSN: 1476-4598