Figure 6
From: Gemcitabine intercellular diffusion mediated by gap junctions: new implications for cancer therapy
Gemcitabine cytotoxic effect on spheroids. A. Spheroid diameters were measured 6 days after gemcitabine treatment for 2 hours or 24 hours with (blue bars) or without AGA (red bars). Spheroid diameters are expressed as percentage of untreated spheroids with or without AGA. Data are the means of five replicates ± S.D. of one representative experiment performed twice. Statistical significance between untreated and gemcitabine-treated cells was evaluated by a Student t-test (**, p < 0.01; NS, p > 0.05): B. SKI-1 viability in spheroids was measured 6 days after gemcitabine treatment for 24 hours. Spheroid viability is expressed as percentage of untreated spheroids with or without AGA (-). Individual data of eight replicates are displayed for each condition. Means are presented as horizontal bars. Statistical significance between untreated (-) and AGA-treated cells was evaluated by a Student t-test (***, p < 0.0001).